recombinant mouse mimecan Search Results


mimecan protein, mouse, recombinant  (TargetMol)
N/A
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proteoglycans mimecan  (R&D Systems)
90
R&D Systems proteoglycans mimecan
FIGURE 6 Proteolytic activity of L. interrogans leptolysin on <t>proteoglycans</t> and plasma fibronectin. (A) Degradation of substrates: <t>mimecan,</t> lumican, biglycan, decorin (0.5 mg), and plasma fibronectin (5 mg) were incubated with leptolysin (0.1 µg) at 37°C for the indicated time points. Cleavage products were subjected to SDS- polyacrylamide gel under reducing conditions, transferred to nitrocellulose membranes, and probed with specific antibodies, or the gel was silver stained (FP). (B) Inhibition of proteolytic activity: before the addition of each substrate, leptolysin (0.1 µg) was incubated with 5 mmol/L 1,10-phenanthroline (lane 4) for 30 min at room temperature. Substrates were added and incubations proceeded for 24 h. Cleavage products were analyzed as described above. Mim (mimecan), Lum (lumican), Bgn (biglycan), Dec (decorin), FP (plasma fibronectin), Sup (Leptospira culture supernatant), Leptol. (leptolysin), Fen (1,10-phenanthroline).
Proteoglycans Mimecan, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+mimecan/Recombinant+Mouse+Mimecan+Protein%2C+CF/pm36081769-135-12-27
Average 90 stars, based on 1 article reviews
proteoglycans mimecan - by Bioz Stars, 2026-09
90/100 stars
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recombinant mouse mimecan  (R&D Systems)
91
R&D Systems recombinant mouse mimecan
FIGURE 6 Proteolytic activity of L. interrogans leptolysin on <t>proteoglycans</t> and plasma fibronectin. (A) Degradation of substrates: <t>mimecan,</t> lumican, biglycan, decorin (0.5 mg), and plasma fibronectin (5 mg) were incubated with leptolysin (0.1 µg) at 37°C for the indicated time points. Cleavage products were subjected to SDS- polyacrylamide gel under reducing conditions, transferred to nitrocellulose membranes, and probed with specific antibodies, or the gel was silver stained (FP). (B) Inhibition of proteolytic activity: before the addition of each substrate, leptolysin (0.1 µg) was incubated with 5 mmol/L 1,10-phenanthroline (lane 4) for 30 min at room temperature. Substrates were added and incubations proceeded for 24 h. Cleavage products were analyzed as described above. Mim (mimecan), Lum (lumican), Bgn (biglycan), Dec (decorin), FP (plasma fibronectin), Sup (Leptospira culture supernatant), Leptol. (leptolysin), Fen (1,10-phenanthroline).
Recombinant Mouse Mimecan, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+mimecan/Recombinant+Mouse+Mimecan+Protein%2C+CF/10__4137_slash_bmi__s7777-25-0-7
Average 91 stars, based on 1 article reviews
recombinant mouse mimecan - by Bioz Stars, 2026-09
91/100 stars
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recombinant mouse mimecan protein cf  (R&D Systems)
N/A
The Recombinant Mouse Mimecan Protein from R D Systems is derived from NS0 The Recombinant Mouse Mimecan Protein has been validated for the following applications Bioactivity
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FIGURE 6 Proteolytic activity of L. interrogans leptolysin on proteoglycans and plasma fibronectin. (A) Degradation of substrates: mimecan, lumican, biglycan, decorin (0.5 mg), and plasma fibronectin (5 mg) were incubated with leptolysin (0.1 µg) at 37°C for the indicated time points. Cleavage products were subjected to SDS- polyacrylamide gel under reducing conditions, transferred to nitrocellulose membranes, and probed with specific antibodies, or the gel was silver stained (FP). (B) Inhibition of proteolytic activity: before the addition of each substrate, leptolysin (0.1 µg) was incubated with 5 mmol/L 1,10-phenanthroline (lane 4) for 30 min at room temperature. Substrates were added and incubations proceeded for 24 h. Cleavage products were analyzed as described above. Mim (mimecan), Lum (lumican), Bgn (biglycan), Dec (decorin), FP (plasma fibronectin), Sup (Leptospira culture supernatant), Leptol. (leptolysin), Fen (1,10-phenanthroline).

Journal: Frontiers in cellular and infection microbiology

Article Title: Leptolysin, a Leptospira secreted metalloprotease of the pappalysin family with broad-spectrum activity.

doi: 10.3389/fcimb.2022.966370

Figure Lengend Snippet: FIGURE 6 Proteolytic activity of L. interrogans leptolysin on proteoglycans and plasma fibronectin. (A) Degradation of substrates: mimecan, lumican, biglycan, decorin (0.5 mg), and plasma fibronectin (5 mg) were incubated with leptolysin (0.1 µg) at 37°C for the indicated time points. Cleavage products were subjected to SDS- polyacrylamide gel under reducing conditions, transferred to nitrocellulose membranes, and probed with specific antibodies, or the gel was silver stained (FP). (B) Inhibition of proteolytic activity: before the addition of each substrate, leptolysin (0.1 µg) was incubated with 5 mmol/L 1,10-phenanthroline (lane 4) for 30 min at room temperature. Substrates were added and incubations proceeded for 24 h. Cleavage products were analyzed as described above. Mim (mimecan), Lum (lumican), Bgn (biglycan), Dec (decorin), FP (plasma fibronectin), Sup (Leptospira culture supernatant), Leptol. (leptolysin), Fen (1,10-phenanthroline).

Article Snippet: Recombinant L. interrogans leptolysin (0.1 mg) was incubated with 0.5 mg of proteoglycans mimecan (# 2949-MC), lumican (# 2846-LU), biglycan (# 2667-CM) and decorin (# 143- DE) (R & D Systems, Inc., Minneapolis, USA), and with 5 μg of plasma fibronectin (Sigma-Aldrich, St. Louis, MO, USA) for up to 4 h in 50 mM Tris HCl, 200 mM NaCl, 10 mM CaCl2 and 0.05% CHAPS, pH 7.4, at 37°C.

Techniques: Activity Assay, Clinical Proteomics, Incubation, Staining, Inhibition